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Figure 7 | BMC Biochemistry

Figure 7

From: Aglycone specificity of Thermotoga neapolitana β-glucosidase 1A modified by mutagenesis, leading to increased catalytic efficiency in quercetin-3-glucoside hydrolysis

Figure 7

Interactions around position 221. The Tn Bgl1A homology model with a cellotetraose ligand with and without residue N221 (blue) mutated to S (yellow). The S mutation allows for recognition of the 6-hydroxyl group of the glucose in the +1 subsite and of the 5-hydroxylgroup of quercetin, by hydrogen bonding to the backbone carbonyl oxygen of residue N291.

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BMC Biochemistry

ISSN: 1471-2091

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