Characterization of a core fragment of the rhesus monkey TRIM5α protein

BMC Biochemistry

Table 1 Expression and solubility of rhesus monkey TRIM5α variants in E. coli

^aThe rhesus monkey TRIM5α variants are designated according to the domains included in the protein: R = RING domain; B = B-box 2 domain; CC = coiled-coil domain; L2 = linker 2 region. All of the TRIM5α constructs were expressed in BL21(DE3)pLysS E. coli using the Champion pET Gateway vector, which adds an N-terminal hexa-histidine tag and a spacer. The inserted fragment of the RBCCL2 protein corresponds to the TRIM5α_rh sequences ¹MASGILL...MEFRLTDA²⁹⁶. The inserted fragment of the RING-BCCL2 protein corresponds to the TRIM5α_rh sequences ¹⁵CPICLE...MEFRLTDA²⁹⁶.
^bThe amount of protein found in the soluble and insoluble (inclusion body) fractions is indicated, with increased levels designated by increasing numbers of plus signs.

ISSN: 1471-2091