Figure 5
From: Characterization of a core fragment of the rhesus monkey TRIM5α protein
Size-exclusion chromatography-light scattering (SEC-LS) analysis of the LLER protein. A. Approximately 300 μg of the purified LLER protein was applied to a Superose 6 HR 10/30 column coupled with an in-line Dawn EOS laser light-scattering apparatus, refractometer and UV detector. The dashed red line represents the light-scattering signal at 90°. The green trace represents the refractive index. The blue trace represents the UV absorption at 280 nm. B. The solid red line indicates the refractometer trace of Peak 1 (P1) and Peak 2 (P2). The "dots" represent the weight-average molecular mass for each slice, measured every second. The results of the analysis performed on the protein in Peak 2 (P2) are shown beneath the figure. The table summarizes the analyses of Peaks 1 and 2.