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Figure 10 | BMC Biochemistry

Figure 10

From: Characterization of a core fragment of the rhesus monkey TRIM5α protein

Figure 10

Trypsin sensitivity of TRIM5α-21R and LLER proteins. The TRIM5α-21R and LLER proteins were incubated at 37°C with a 1:150 (weight/weight) solution of trypsin in 25 mM bicarbonate buffer, pH 8 for the indicated times. The reaction was stopped by adding soybean trypsin inhibitor (STI) and the reaction mixtures were boiled in Laemmli buffer. The mixtures were then analyzed by SDS-PAGE and the gels were stained with Coomassie Blue.

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