Activities were measured at 30°C at pH 7.0 in a continuous coupled enzyme assay in the presence of 50 mM Pi and 24 g/l maltodextrin (Agenamalt 19DE). The kcat value was calculated from the specific activity (U/mg) assuming a molecular mass of 90.6 kDa for the enzyme subunit. PLP values have S.D. of < 20% (N = 3) while activity values have S.D. of < 10% (N = 4).