| PLP | activity |
---|
| [μM/μM protein] | [U/mg] | kcat [s-1] |
---|
wild-type | 0.7 | 31 | 47 |
T28A | 0.6 | 29 | 44 |
K31A | 0.6 | 29 | 44 |
S174A | 0.7 | 29 | 44 |
R141A | 0.7 | 27 | 40 |
- Activities were measured at 30°C at pH 7.0 in a continuous coupled enzyme assay in the presence of 50 mM Pi and 24 g/l maltodextrin (Agenamalt 19DE). The kcat value was calculated from the specific activity (U/mg) assuming a molecular mass of 90.6 kDa for the enzyme subunit. PLP values have S.D. of < 20% (N = 3) while activity values have S.D. of < 10% (N = 4).