Figure 7
Detail of the 1H-15N HSQC spectra corresponding to the titrations of 4.1RCTD64 with NuMA1 peptides at pH 4.9. A and B in one hand and C and D in the other show the same regions of the spectra. A and C: Spectra of 4.1R-CTD64 alone (black) or in presence of 20 to 1 ratio of N1I (sequence 1785-1810 of NuMA1) (blue). B and D: Spectra of 4.1RCTD64 alone (black) or in presence of 20 to 1 ratio of N1IP (N1I phosphorylated) (red). Note the drastic changes in the signal intensity of T51 and T59 after titration with N1IP, of I11 after titration with N1I, or of I38 after titration with both peptides.