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Figure 6 | BMC Biochemistry

Figure 6

From: NMR characterisation of the minimal interacting regions of centrosomal proteins 4.1R and NuMA1: effect of phosphorylation

Figure 6

Titration of 4.1RCTD64 with N1O peptide at pH 4.9 and with N1I at pH 4.0. Titration of 15N labelled 4.1R-CTD64 followed by 1H-15N HSQC NMR spectra at 25°C. A: Titration with N1O (sequence 1776-1796 of NuMA1) at pH 4.9. B: Titration with N1I (sequence 1785-1810 of NuMA1) at pH 4.0. In both cases black lines belong to the NMR spectra of 4.1R-CTD64 alone and red lines to the spectra in presence of NuMA1 peptides (ratio 1:20).

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