Figure 2

CD and ¹H-¹⁵N HSQC spectra of 4.1R domains. A: CD spectra of the C-terminal domain of 4.1R. B: CD spectra of the 4.1R-CTD64 domain of 4.1R. Samples for CD were 5 mM in 10 mM Na₂HPO₄ at pH 6.5 (4.1R C-terminal domain) or pH 4.9 (4.1R-CTD64). C: ¹H-¹⁵N HSQC spectra of the C-terminal domain of 4.1R at pH 6.5 (0.6 mM protein in H₂O 90%/D₂O 10% at pH 6.5). D: ¹H-¹⁵N HSQC spectra of the 4.1R-CTD64 domain at pH 4.9 (0.1 mM protein in Na₂HPO₄ 100 mM in 90% H₂O/10% D₂O pH4.9) with signal assignments. Note that the spectral width in D is smaller than in C to facilitate the visualization of the assignments.