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Figure 4 | BMC Biochemistry

Figure 4

From: Glyceraldehyde-3-phosphate dehydrogenase is largely unresponsive to low regulatory levels of hydrogen peroxide in Saccharomyces cerevisiae

Figure 4

Characterization of the antibodies used for GAPDH detection. Representative (n≥3) two-dimensional analysis of Tdh1p, Tdh2p and Tdh3p (only the region with the 3 GAPDH isoenzymes is shown) S. cerevisiae cells with immunodetection made by Western blot as described in materials and methods are shown. (A) wt stationary-phase cells with identification using mouse anti-GAPDH (Chemicon MAB374). Identification using rabbit anti-GAPDH was performed in (B) wt exponential-phase cells, (C) wt stationary-phase cells, and (D) tdh1 Δ stationary-phase cells.

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