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Figure 3 | BMC Biochemistry

Figure 3

From: Glyceraldehyde-3-phosphate dehydrogenase is largely unresponsive to low regulatory levels of hydrogen peroxide in Saccharomyces cerevisiae

Figure 3

Low H 2 O 2 concentrations do not inactivate GAPDH in S. cerevisiae. GAPDH activity in exponential-phase cells (A, B) or stationary-phase cells (C) treated with either the indicated steady-state H2O2 concentrations for 60 min or (B) with a 2 mM H2O2 bolus addition for 30 min was measured in the presence (closed bars) and in the absence of DTT (dashed bars) in the assay medium. The difference of the activity measured in the presence and in the absence of DTT is a measure of reversible GAPDH thiol oxidation. The values are the mean ± standard deviation of 3≤n < 10 independent experiments. *P < 0.05 vs DTT; #P < 0.01 vs DTT; **P < 0.001 vs control.

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BMC Biochemistry

ISSN: 1471-2091

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