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Figure 4 | BMC Biochemistry

Figure 4

From: The Fer tyrosine kinase regulates interactions of Rho GDP-Dissociation Inhibitor α with the small GTPase Rac

Figure 4

Fer interferes with binding of RhoGDIα and Rac. (A) GST-RhoGDIα was pre-incubated with Rac, after which GST-Fer (G-Fer) or GST (G) was added and GST fusion proteins were pulled down with glutathione agarose, followed by kinase reactions. (B) GST-RhoGDIα was pre-incubated with GST-Fer or GST in kinase buffer, after which Rac was added and RhoGDIα was immunoprecipitated. 50 pmol of RhoGDIα and Rac1, with 25 pmol of GST and GST-Fer were used. Blots were reacted with Rac1 antibodies or pY20, then stripped and reacted with GST antibodies. This result is representative of two independent experiments.

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