Table 2 Class-specific inhibitors effect on pepG1 activity
From: Identification and characterization of a bacterial glutamic peptidase
pepG1 was incubated for 30 min with the below inhibitors at pH 4.0 (10 min, pH 4.5 for E-64). The remaining activity was assayed at 37°C. | |||
|---|---|---|---|
Inhibitor | Class-specific inhibitor | Concentration (mM) | Relative activity |
Pepstatin | Aspartic | 0.005 | 0.92 |
EDTA | Metallo | 10 | 0.93 |
PMSF | Serine | 10 | 0.94 |
E-64 | Cysteine | 1 | 0.99 |