Figure 6

Characterization of pepG1. A. Effect of pH on pepG1 activity. The maximum activity at 37°C towards AZCL-collagen was obtained at a broad plateau around pH 3.0 and set at 100%. B. Determination of temperature optimum for pepG1. The maximum activity towards AZCL-collagen was observed at 60°C, pH 4.0 and set at 100%. C. pH stability of pepG1. pepG1 was diluted and incubated in assay buffer pH 2-12 for two hours at 37°C. pH was then adjusted to pH 4.0 and activity was measured at 37°C. D. Temperature stability of pepG1. pepG1 was incubated at 50°C (black), 60°C (grey) and 70°C (light grey) for up to one hour, cooled to 4°C on ice and assayed at 37°C, pH 4.0. Stability is measured relative to samples incubated on ice.