Figure 6

Enriched Tetrahymena nuclease shows sequence-dependant cleavage. Activity of Tetrahymena nuclease (30 ng) and Exo1 (1 fmol or 0.04 ng) with various unique sequences that end in all combinations of two terminal nucleotides, either unphosphorylated (A) or phosphorylated (B) were tested. Reactions were performed for 20 min and resolved on 10% urea denaturing polyacrylamide gels. All assays were run and performed on the same day, and the time for exposure to phosphorimager screens was the same. Substrates used are: S1:S18* (lanes 1-3), S3:S20* (lanes 4-6), S35:S57* (lanes 7-9), S36:S58* (lanes 10-12), S37:S59* (lanes 13-15), S38:S60* (lanes 16-18), S39:S61* (lanes 19-21), S40:S62* (lanes 22-24), S41:S63* (lanes 25-27), S42:S64* (lanes 28-30), S43:S65* (lanes 31-33), S44:S66* (lane 34-36), S45:S67* (lanes 37-39), S46:S68* (lanes 40-42), S47:S69* (lanes 43-45), S48:S70* (lanes 46-48), S49:S71* (lanes 49-51), S50:S72* (lanes 52-54), S51:S73* (lanes 55-57), S52:S74* (lanes 58-60), S53:S75* (lanes 61-63).