Figure 2

Positioning of DnaK-binding peptides in preproinsulin. Lines below the amino acid sequence represent the 50 immobilized 13-mer preproinsulin peptides used in the screening approach. Peptides with low or high DnaK-retention are shown as dashed or straight lines, respectively. Hatched boxes indicate the cores of the DnaK-binding regions as deduced from the 13-mer peptides showing peak values of bound DnaK. The solid black box indicates peptide B11-23 with high DnaK affinity and the open box indicates peptide B18-30 wit low DnaK affinity.