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Figure 7 | BMC Biochemistry

Figure 7

From: Soluble perlecan domain i enhances vascular endothelial growth factor-165 activity and receptor phosphorylation in human bone marrow endothelial cells

Figure 7

PlnDI/VEGF 165 mixtures enhance VEGFR-2 (Tyr-951) phosphorylation. Human bone marrow endothelial cells without cell surface HS were incubated with PlnDI/VEGF165 mixtures for either 0, 2, 5, 10, or 20 min. Cell lysates were analyzed for VEGFR-2 (A and B) or Akt (C) phosphorylation by Western blot using anti-phospho and total VEGFR-2 (tyrosine residue 951) and Akt specific antibodies. (B) PlnDI/VEGF165 enhanced VEGFR-2 phosphorylation (at min 2.5): Requirement of CS and HS chains on PlnDI. Horizontal bars represent the densitometric scanning of bands outlined in rectangular boxes. GAPDH was assessed as a loading control. Data are presented as the mean of three independent experiments ± SEM. (*) different from 0 min; (γ) different from VEGF165; (φ) different from PlnDI+VEGF165. p < 0.05.

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