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Figure 5 | BMC Biochemistry

Figure 5

From: Soluble perlecan domain i enhances vascular endothelial growth factor-165 activity and receptor phosphorylation in human bone marrow endothelial cells

Figure 5

In the absence of cell surface heparan sulfate increased PlnDI concentrations restore synergy with VEGF 165 . Human bone marrow endothelial cells, in suspension, were treated with a heparinase cocktail, washed, then incubated with PlnDI [12.5 μg/ml] +/- VEGF165 [20 ng/ml] for 30 minutes prior to seeding in wells pre-coated with growth factor reduced Matrigel. After 18 h cells were fixed, stained with SYTO13, and tube length quantified. Both PlnDI and VEGF165 enhance capillary tube-like formation. A two fold increase in PlnDI concentration [25 μg/ml] is required for synergy with VEGF165. Data are presented as the mean lengths from nine areas of triplicate determinations ± SEM. (*) different from media; (γ) different from VEGF165. p < 0.05.

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