Figure 4
Exogenous PlnDI enhances capillary tube-like formation. Human bone marrow endothelial cells were seeded in 96 well plates pre-coated with growth factor reduced Matrigel and treated with PlnDI pre-incubated +/- VEGF165. Tube length quantification was performed after 18 h on fixed cells, stained with SYTO13. Effect of VEGF165 (A), and PlnDI (B) dose on capillary tube-like formation. (C) PlnDI [12.5 μg/ml] enhanced tube-like formation: Requirement of CS and HS chains. (D) PlnDI/VEGF165 enhanced tube-like formation: Requirement of CS and HS chains on PlnDI. (E) Effect of heparin/VEGF165 mixtures on capillary tube-like formation. (F) Representative images of tube-like formation in panel A. Data are presented as mean lengths of nine areas from triplicate determinations ± SEM. (*) different from media; (ψ) different from VEGF165 at 5, 10 and 80 ng; (π) different from PlnDI 6 and 25 μg; (δ) different from PlnDI; (γ) different from VEGF165. p < 0.05.