Figure 3

VEGF ₁₆₅ binding to PlnDI in solution is pH dependent. PlnDI pre-incubated with VEGF₁₆₅ in 25 mM HEPES (pH 6.0, 7.0, or 8.0) was immobilized on nitrocellulose. VEGF₁₆₅ bound to PlnDI was detected with VEGF specific monoclonal antibodies. (A) pH dependent binding of VEGF₁₆₅ to PlnDI. (B) The requirement of CS and HS chains for VEGF₁₆₅ binding to PlnDI (pH 7.0). Data are presented as mean specific density values (DV) of triplicate determinations ± SEM. Specific binding was determined by subtracting the background binding of VEGF₁₆₅ to nitrocellulose from total bound. (*) different from pH 8.0; (**) different from pH 7.0; (φ) different from PlnDI+VEGF₁₆₅; (θ), different from PlnDI+Hepase+VEGF₁₆₅. p < 0.05.