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Figure 1 | BMC Biochemistry

Figure 1

From: Soluble perlecan domain i enhances vascular endothelial growth factor-165 activity and receptor phosphorylation in human bone marrow endothelial cells

Figure 1

SDS-PAGE and Western blot analysis of PlnDI. (A) Coomassie blue staining; (B) Alcian blue staining: lane 1, undigested PlnDI; lane 2, heparinase cocktail treated PlnDI; lane 3, chondroitinase ABC treated PlnDI; lane 4 heparinase cocktail and chondroitinase ABC treated PlnDI. (C) Western blot analysis with PlnDI and (D) heparan sulfate stub (3G10) specific antibodies respectively: Lane 1, undigested PlnDI; lane 2, heparinase cocktail and chondroitinase ABC treated PlnDI. Heparinase cocktail is a mixture of heparinases I, II, and III. Arrows indicate the protein core of PlnDI released after heparinase cocktail and chondroitinase ABC digestion. α,β,γ,δ, ϕ indicate the migration positions of heparinases I, II, III, chondroitinase ABC, and BSA respectively. Bracket in panel C denotes immunoreactive products released following incomplete digestion.

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BMC Biochemistry

ISSN: 1471-2091

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