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Figure 5 | BMC Biochemistry

Figure 5

From: Regulation of Ack1 localization and activity by the amino-terminal SAM domain

Figure 5

Increasing local concentrations of purified Ack1 kinase domain at the surface of lipid vesicles increases catalytic activity. The catalytic activity of Ack1 kinase domain towards a peptide was measured as in Figure 4. The activities of purified Ack1 kinase domain were measured in solution (no ves) or with vesicles containing 0 or 5 mole % of DOGS-NTA-Ni. In one experiment (5% + Im), the reaction was carried out in the presence of 500 mM imidazole. The enzyme concentration was 250-500 nM, peptide substrate was used at 1 mM, and the Ni-NTA concentration was kept constant at 30 μM. The figure shows the average results of nine experiments performed in triplicate. Errors bars indicate standard error.

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