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Figure 3 | BMC Biochemistry

Figure 3

From: Regulation of Ack1 localization and activity by the amino-terminal SAM domain

Figure 3

Quantitative immunofluorescence analysis. A, Cos7 cells expressing wild type or truncated forms of Ack1 were prepared for immunofluorescence using anti-phospho-Ack1 (pY284) and anti-HA antibodies. Top row: HA staining; middle row: pY284 staining; bottom row: merged images. The images in panel A were taken from the set used for quantitation and statistics in panels B and C. B, Immunofluorescence (anti-pY284 and anti-HA) was measured in three separate experiments (30 cells per experiment). The ratio of pY284Ack1/HA in each cell was calculated and normalized with respect to WT Ack1. Acquisition parameters were the same for all cells, and images shown as well as those used for quantitation were not further processed. A scatter plot for one representative experiment, with a line at the mean is shown. C, Bar graph showing the combined data of the three experiments. The data were compared using a one-way ANOVA test (P < 0.0001), followed by a Tukey's test of honest significant differences. All groups were significantly different from each other; P < 0.001 for all pairings, with the exception of KD vs. K158R (P < 0.01). Errors bars indicate standard error.

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