Figure 2
From: Regulation of Ack1 localization and activity by the amino-terminal SAM domain
Localization of Ack1 proteins. Cos7 cells co-expressing wild-type or mutant HA-tagged Ack1 along with a membrane targeted form of GFP (PM-GFP) [40] were prepared for immunostaining and confocal microscopy as described in Materials and Methods. Ack1 proteins were detected with anti-HA antibodies. Anti-HA; left panels; PM-GFP, middle panels; merged images, right panels. Scale bar: 10 microns.