Figure 1

The N-terminus of Ack1 is required for autophosphorylation in cells. A, constructs used in this study: WT, full length Ack1 (accession # Q07912); ΔNt, deletion of the N-terminal 110 amino acids; NKD, amino terminus plus kinase domain (residues 1-381); KD, kinase domain alone (residues 111-381). B, Western blot analysis. Lysates from Cos7 cells expressing wild type or truncated forms of Ack1 were probed with anti- phospho Ack1 (pY284), anti-HA, and anti-phosphotyrosine antibodies as indicated. The figure is representative of five experiments. C, densitometry readings were taken from all five experiments and the average was plotted in a bar graph. Error bars indicate standard error.