Figure 6
Time-course of GlcNAc 6 hydrolysis catalyzed by Vh Nag2. Upper panels: Experimental time-courses obtained by HPLC determination. The early stage of the reaction time-course is shown in the left panel, and the later stage is shown in the right panel. The reaction conditions are described in the text. Lower panels: Calculated time-courses best fitted to the experimental ones. The calculation was conducted using the rate constants and binding free energy values listed in Table 2.