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Figure 4 | BMC Biochemistry

Figure 4

From: Novel β-N-acetylglucosaminidases from Vibrio harveyi 650: Cloning, expression, enzymatic properties, and subsite identification

Figure 4

Time course of chitooligosaccharide hydrolysis by TLC. A reaction mixture (20 μl), containing 800 ng Vh Nag2 and 2.5 mM of A) GlcNAc2; B) GlcNAc3; C) GlcNAc4; D) GlcNAc5; or E) GlcNAc6 in 0.1 M phosphate buffer, pH 7.0, was incubated at various times at 37°C, and then analyzed by TLC. Sugar products were detected with aniline-diphenylamine reagent. Lanes: std, a standard mix of GlcNAc1-6; 1-7, incubation at 1, 5, 10, 15, 30 min, 3 h and 18 h, respectively; and 8, substrate control.

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