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Figure 2 | BMC Biochemistry

Figure 2

From: Novel β-N-acetylglucosaminidases from Vibrio harveyi 650: Cloning, expression, enzymatic properties, and subsite identification

Figure 2

Physical properties of V. harveyi GlcNAcases. A) SDS-PAGE analysis of Vh Nag1 and Vh Nag2 expressed in E. coli and purified by Ni-NTA agarose chromatography. Std: low molecular weight protein markers. B) pH/activity profiles of Vh Nag1 and Vh Nag2. GlcNAcase activity was assayed at different pH values from 2.5 to 9.5 at 37°C for 10 min using p NP-GlcNAc as substrate

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