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Figure 7 | BMC Biochemistry

Figure 7

From: Identification of inhibitors of Plasmodium falciparum phosphoethanolamine methyltransferase using an enzyme-coupled transmethylation assay

Figure 7

Amodiaquine reduced PfPMT-dependent PtdCho levels in yeast. (A) Phospholipid analysis of pem1Δpem2Δ-pYes2.1 and pem1Δpem2Δ-PfPMT strains grown in minimal medium containing 4% galactose and 2 mM ethanolamine. The lipids were extracted, separated by 2-D TLC and stained with iodine vapor. (B) Each lipid was recovered from the TLC plate and quantified by measuring phosphorous. The graph is the percentage of total lipid phosphorous in each lipid fraction. PtdCho-phosphatidylcholine; PtdEtn- phosphatidylethanolamine; PtdSer- phosphatidylserine; PtdIns- phosphatidylinositol. The data are represented as the means +/- S.D. of three independent experiments.

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