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Figure 1 | BMC Biochemistry

Figure 1

From: Identification of inhibitors of Plasmodium falciparum phosphoethanolamine methyltransferase using an enzyme-coupled transmethylation assay

Figure 1

Schematic description of the enzyme-coupled assay for measuring PfPMT activity. PfPMT catalyzes the conversion of 1 molecule of P-EA and 3 molecules of SAM to form 1 molecule of phosphocholine and 3 molecules of SAH. SAH is hydrolyzed to adenine and S-ribosylhomocysteine via SAHN nucleosidase. The deamination of adenine into hypoxanthine by adenine deaminase is associated with a decrease in absorbance at 265 nm that can be monitored continuously using UV plate reader.

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BMC Biochemistry

ISSN: 1471-2091

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