Table 1 Primers used for analysis of gene expression by real time quantitative RT-PCR
From: Sex-different hepatic glycogen content and glucose output in rats
Accession no. | Gene | Gene name | Forward primer (5'-3') | Reverse primer (5'-3') |
|---|---|---|---|---|
NM_017340 | ACOX1 | acyl-coenzyme A oxidase 1 | AGCTGTGCTGAGGAACCTGT | CTGGTGGATGCCTTTGACTT |
NM_031559 | CPT-1a | carnitine palmitoyltransferase 1a | AAGGTGCTGCTCTCCTACCA | TACCTGGAATCTGTGAGGCC |
NM_139192 | SCD1 | stearoyl-coenzyme A desaturase 1 | GATATCCACGACCCCAGCTC | TACCTTATCAGTGCCCTGGG |
NM_017332 | FAS | fatty acid synthase | CTTTGTGGCCTTCTCCTCTG | GCAGTTTTGTGCTGGTTGAG |
NM_199115 | Angptl4 | angiopoietin-like 4 | CAGGCTACCACCCTGTTGAT | TGGACAGAGAAGAAGCCCAT |
NM_001024743 | UGP2 | UDP-glucose pyrophosphorylase 2 | GGTTTGCTCGACACCTTCAT | TACGAAGGCAAACTGAGGCT |
NM_013098 | G6Pase | glucose-6-phosphatase | CTACCTTGCGGCTCACTTTC | GACCTCCTGTGGACTTTGGA |
NM_198780 | PEPCK | phosphoenolpyruvate carboxykinase | CCCAGGAGTCACCATCACTT | GTGTCCCCCTTGTCTACGAA |
NM_012571 | GOT1 | glutamate oxaloacetate transaminase 1 | TCCAAGAACTTCGGGCTCTA | GGAGTGGAAAGGAAACGTGA |
NM_022402 | Arbp | acidic ribosomal phosphoprotein Pθ | CAGCAGGTGTTTGACAATGG | AAAGGGTCCTGGCTTTGCTC |