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Figure 5 | BMC Biochemistry

Figure 5

From: Molecular characterization of tlyA gene product, Rv1694 of Mycobacterium tuberculosis: A non-conventional hemolysin and a ribosomal RNA methyl transferase

Figure 5

(A) Immuno-fluorescence localization of Rv1694 on E. coli: E. coli transformed with Rv1694 or pET28a+ was immunostained with immune rabbit serum followed by detection with Cy2 conjugated goat anti-rabbit IgG (green fluorescent). Left side of the panels show the Rv1694 localization (Green), middle panels show the DAPI stained bacteria (Blue) and right panels show the merged images. (B) Inhibition of contact dependent hemolysis by Immune rabbit serum: Rv1694 transformed E. coli was incubated with and without pre-immune/immune rabbit serum as described for Figure 4A. Error bars represent standard deviation of two independent experiments.

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BMC Biochemistry

ISSN: 1471-2091

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