Figure 7

Transcript analysis of genes involved in the methylcitrate cycle and β-oxidation of fatty acids in A. parasiticus strains. For growth conditions and RT-PCR methods, see Methods. R.E., relative expression was calculated as shown in Figure 6. CS, citrate synthase AFLA_007020; 2-MCS, 2-methylcitrate synthase AFLA_049290; 2-MCD, 2-methylcitrate dehydratase AFLA_056350; echA, a short chain enoyl-CoA hydratase AFLA_043610; foxA, an enoyl-CoA hydratase/hydroacyl-CoA dehydrogenase AFLA_041590. The number in parenthesis indicates the expected size of PCR product obtained using genomic DNA.