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Table 2 Purification of AoSOX1 from the T. reese i culture medium.

From: Secreted fungal sulfhydryl oxidases: sequence analysis and characterisation of a representative flavin-dependent enzyme from Aspergillus oryzae

 

Volume

(ml)

Total activity b

(10 6 nkat)

Total protein

(mg)

Specific

activity

(10 6 nkat mg -1 )

Purification

factor

Activity

yield

(%)

Cell free culture mediuma

60

311

324

0.96

1

100

Hitrap DEAE FF

108

261

13

20.20

21

84

Superdex 75 HR

8

210

11

19.97

21

68

  1. a The cell-free culture medium (220 ml) was concentrated and buffer exchanged.
  2. b Enzymatic activity was measured with the HVA-peroxidase coupled assay.