Figure 3From: Secreted fungal sulfhydryl oxidases: sequence analysis and characterisation of a representative flavin-dependent enzyme from Aspergillus oryzaepH optimum and temperature stability of AoSOX1. Sulfhydryl oxidase activity was measured with the HVA-peroxidase coupled assay on glutathione 5 mM at room temperature to determine (a) the pH optimum and (b) temperature stability after 30 minutes (filled square), 1 hour (filled circle), 2 hours (filled triangle) and 24 hours (filled inverted triangle) of incubation at different temperatures.Back to article page