Figure 2From: Secreted fungal sulfhydryl oxidases: sequence analysis and characterisation of a representative flavin-dependent enzyme from Aspergillus oryzaeAoSOX1 molecular characterisation. (a) SDS PAGE analysis (12%, Tris/HCl) showing the T. reesei culture medium of the parental strain (lane 2, 80 μg), of the AoSOX1 expressing strain (lane 3, 80 μg), purified native protein (5 μg and 10 μg,, lane 4 and 6, respectively) and the deglycosylated protein (10 μg, lane 7). Lanes 1 and 5 contain molecular weight markers. (b) Identification by peptide mass fingerprinting of the purified AoSOX1 after in-gel digestion with trypsin. The sequences and masses of the peptides matching with the AoSOX1 sequence are listed and underlined in the protein sequence. Asparagine residues belonging to potential N-glycosylation sites are in bold.Back to article page