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Figure 6 | BMC Biochemistry

Figure 6

From: Na+-stimulated ATPase of alkaliphilic halotolerant cyanobacterium Aphanothece halophytica translocates Na+ into proteoliposomes via Na+ uniport mechanism

Figure 6

Dependence of Na+uptake on Na+and ATP. Na+ uptake into reconstituted proteoliposomes was measured in 20 mM Tris-HCl pH 7.6 containing 5 mM MgCl2 and at various concentrations of 22NaCl ( 80 μCi mmol-1) (A) and ATP (B). The uptake reaction was started by the addition of 4 mM ATP (Tris salt). Insets in (A) and (B) show double-reciprocal plots of Na+ uptake versus NaCl and ATP concentration, respectively.

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