Figure 7

Thioredoxins reduce glutathionylated proteins as cells resume exponential phase from stationary phase growth. (A) Thioredoxins are required to reduce glutathionylation during the transition from stationary phase to exponential phase growth. Stationary phase cells of the wild-type and thioredoxin mutant strains were inoculated into fresh SD media and protein bound (GSSP) glutathione levels determined after two hours growth. Values shown are the means of at least three independent determinations and are given in nmol/ml/A₆₀₀. (B) Thioredoxin mutants are delayed in the exit from stationary phase. Stationary phase cells of the wild-type and thioredoxin mutant strains were inoculated into fresh SD media and growth monitored by following A₆₀₀.