Figure 2

Analysis of mitochondrial gene expression and genome copy number. Expression analysis of mitochondrial encoded genes (A) and nuclear encoded mitochondrial transcription factors (B). Total RNA isolated from control and SIN3-deficient cells (KD1, black bars, and KD2, gray bars) was used to generate cDNA. qPCR was performed using the cDNA as a template and primer pairs for the genes indicated along the X-axis. Relative levels of gene expression are indicated. Error bars represent standard error. A, n = 7 for KD1 and n = 5 for KD2, p < 0.05 for all data. B, n = 4, *p < 0.05. (C) Mitochondrial DNA copy number is unaltered following loss of SIN3. DNA was isolated from control and SIN3-deficient cells. qPCR was performed using the DNA as a template and primer pairs for both nuclear and mitochondrial genome sequences as indicated along the X-axis. Pro-cyt-c-p amplifies the promoter region of Cyt-c-p. Relative copy number is indicated. The nuclear gene glutathione transferase (CG11784) was used for normalization. Error bars represent standard error (n = 3).