Figure 1

Expression of nuclear encoded genes involved in multiple mitochondrial processes is up-regulated following loss of SIN3. SIN3-deficient cells were generated by RNAi methodology using dsRNA generated against different regions of the SIN3 transcript KD1 (black bars) or KD2 (gray bars). (A) Western blot analysis of whole cell extracts prepared from control or SIN3-deficient cells as indicated. The blots were probed with antibody to SIN3 or to β-tubulin as a loading control. (B) qPCR was performed using cDNA prepared from control and SIN3-deficient cells as a template and primer pairs for the genes indicated along the Y-axis. Relative levels of gene expression are indicated. *Genes with decreased expression in microarray. **Genes with no expression level change in microarray. Genes are grouped according to process: I-glycolysis, II-citric acid cycle, III-fatty acid oxidation, IV-oxidative phosphorylation, V-mitochondrial biogenesis, VI-reactive oxygen species (ROS) pathways, VII-heme synthesis, and VIII-transporter. Error bars represent standard error (n = 3-6 for KD1 and n = 3-5 for KD2). p ≤ 0.05 for all data.