Figure 7
From: Identification of archaeal proteins that affect the exosome function in vitro
Binding of PaNip7 to the Pa-exosome complexes analyzed by size exclusion chromatography. 12.5 μg of PaNip7 labeled with FITC was incubated with 25 μg of the exosome complexes for 30 minutes at 25°C. Protein complexes were subjected to size exclusion chromatography. Fluorescence emission was detected at 520 nm. (A) separate proteins. (B) PaNip7 incubated with the exosome complexes. Molecular mass standards used were aldolase (158 kDa) and chymotrypsin (25 kDa).