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Table 3 xDYRK1B kinase activity in Xenopus laevis oocytes

From: Development of a sensitive non-radioactive protein kinase assay and its application for detecting DYRK activity in Xenopus laevis oocytes

 

Phosphorylated peptide (pmol)

IP (antibody)

ELISA-based assay (n = 3)

Radiometric assay (n = 1)

 

1

2

3

Mean ± S.D.

 

Pre-immune

0.30

1.23

1.27

0.93 ± 0.55

0.15

αDYRK1B

6.19

8.19

11.34

8.57 ± 2.60

3.37

  1. Immunocomplex kinase assays were performed with lysates of about 100 μl settled oocytes per assay. Results of three independent ELISA assays (1-3) with tau207-219 and one radiometric assay with DYRKtide are reported.
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BMC Biochemistry

ISSN: 1471-2091

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