Development of a sensitive non-radioactive protein kinase assay and its application for detecting DYRK activity in Xenopus laevis oocytes

Table 2 Characteristics of the two Xenopus DYRK1 isoforms

	xDYRK1A	xDYRK1B
Database entries
XenBase^a gene symbol	dyrk1a	dyrk1a.2
UniProt^b	Q2TAE3	Q7ZXV4
UniGene^c	Xl.29801	Xl.5747
Expression clones used
IMAGE clone	5542675	4724858
GenBank acc.	BC110968	BC044104
Protein characteristics
molecular mass	84 kDa	76 kDa
histidine repeat^d	His₁₁	absent
Sequence identity with hDYRK1A
catalytic domain	97%	90%
C-terminal domain	85%	<30%
Sequence identity with hDYRK1B
catalytic domain	84%	87%
C-terminal domain	<30%	59%

^a http://www.xenbase.org; ^b http://www.uniprot.org^c http://www.ncbi.nlm.nih.gov/unigene
^d Human DYRK1A contains a repeat of 13 consecutive histidines, human DYRK1B contains no histidine repeat

ISSN: 1471-2091