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Figure 7 | BMC Biochemistry

Figure 7

From: Thermal stability, pH dependence and inhibition of four murine kynurenine aminotransferases

Figure 7

Transamination activity of mouse brain crude protein extract towards different α-ketoacids. The reaction mixture consists of 5 mM kynurenine, 2 mM α-keto acid, 40 μM PLP, 20 μg brain crude protein extract in 100 μL 100 mM phosphate buffer, pH 7.5. The mixture was incubated at 38°C for 2 h and the reaction was stopped by adding an equal volume of 0.8 M formic acid. Measurement of KYNA was performed by HPLC with fluorometric detection at Exc. 340 nm and Em. 398 nm.

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