Table 1 List of oligonucleotides used in this study.
From: Activity of the acyl-CoA synthetase ACSL6 isoforms: role of the fatty acid Gate-domains
Name | sequence 5' to 3' |
|---|---|
Hs.ACSL6-130/fwd | AGCTAGCTTCACTCACCGGCCAAAGGCC |
Hs.ACSL6-end/rev | GGAAGCTTCACATGGAGATTGAGTAAAGCTCTTC |
L6-v3-Nhe | ACTATAGCTAGCCAGACACAGGAGATCCTG |
L6-Gate/rev | AAAGGGTCGACCTCTGTCACTTTCAGAAAGCC |
L6-Gate/fwd | AAAGGGTCGACTCTGTCGTCTATTGCCACGGAG |
Y319W-rev | ACATGTGTGCTAAAGGCAACCAGGAAATGTGCACATCCGCA |
Y319A-rev | CATGTGTGCTAAAGGCAAAGCGGAAATGTGCACATCCGCA |
Y319F-rev | ATGTGTGCTAAAGGCAAAAAGGAAATGTGCACATCCG |
F319W | GATGTGCTCATCTCCTGGCTGCCTCTGGCTCACA |
F319A | CGATGTGCTCATCTCCGCCCTGCCTCTGGCTCAC |
F319Y-rev | ATGTGAGCCAGAGGCAGATAGGAGATGAGCACATCG |
F/Y-L316H-rev | GGCAGATAGGAGATGTGCACATCGTCCTGTC |
F-L316H-rev | GGCAGGAAGGAGATGTGCACATCGTCCTGTC |
Y-H316L | CACTTGTGCGGATGTGCTCATTTCCTATTTGCCTT |
Y/F-H316L | CACTTGTGCGGATGTGCTCATTTCCTTTTTGCCTT |
Cola-BamH | ACTATAGGATCCGCAGACACAGGAGATCCTGAGG |
Cola-Pml | ACTATACACGTGCAGACACAGGAGATCCTGAGG |
Cola-Hind | CTATGCAAGCTTTCACATGGAGATTGAGTAAAGC |
Cola-Xho | CTATGCCTCGAGCATGGAGATTGAGTAAAGCTC |
ACSL6-EagI | TACTCGGCCGAGACACAGGAGATCCTGAGG |
ΔN-EagI | TACTCGGCCGCTCACCGGCCAAAGGCCTTGC |
HA-fwd | ATGTACCCATACGATGTTCCAGATTACGCTAGAT |
HA-rev | ATCTAGCGTAATCTGGAACATCGTATGGGTACAT |