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Figure 2 | BMC Biochemistry

Figure 2

From: Activity of the acyl-CoA synthetase ACSL6 isoforms: role of the fatty acid Gate-domains

Figure 2

Acyl-CoA synthetase activity of ACSL6 isoforms. Activity rate values (nmol of C18:1-CoA formed/mg of membrane proteins per min) of isoform 1 (Y-Gate), isoform 2 (F-Gate) and their respective N-terminus truncated forms (ΔN-Y-Gate, ΔN-F-Gate) are represented as function of the concentration of the substrate oleic acid. For each concentration of (14C)-C18:1-OH, rates were obtained by measurements at 30°C of the formation of C18:1-CoA at 1, 2 and 3 minutes after initiation of the reaction with 3 μg of membrane proteins for isoform 1, 1 μg for isoform 2 and 0.25 μg for the 2 ΔN-truncated forms. Each value represents the average of 4 different measurements and the standard deviation is indicated as error bars. Inset top: Activity of isoform 1 was low and is shown with a different y axis. Inset bottom: Kinetic parameters [K m (μM) and Vmax (nmol/mg.min)] were calculated from these data using Prism 5.0 program (see Methods) and are shown in the table.

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