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Figure 1 | BMC Biochemistry

Figure 1

From: Activity of the acyl-CoA synthetase ACSL6 isoforms: role of the fatty acid Gate-domains

Figure 1

Human ACSL6 isoforms and truncated constructs. A. Cartoon representation of the three spliced isoforms, and of the four truncated constructs studied in this work. Isoform 1 and 2 represent spliced forms with a Gate-domain containing a tyrosine (Y) or a phenylalanine (F) at position 319, respectively. Isoform 3 has a short internal deletion at the N-terminus, and has no Gate-domain. Numbers on the right of each of the construct represent the acyl-CoA synthetase activity rate values (nmol acyl-CoA formed per mg proteins per min) obtained with oleic acid (6 μM), and the standard deviations of 3 measurements are given in parenthesis. Note that for isoform 3 and the two constructs ΔGate and ΔN-ΔGate (see text), activity values were as low as the values obtained with control cells transformed with the empty vector alone (values of ≈0.5) and could not be reliably detected (not detected, n.d.). B. Amino acids alignment of the N-terminus (top) and of the Gate-domain motif (bottom). A predicted trans-membrane spanning segment (amino acid 21 to 42) at the N-terminus is indicated for isoform 1 and 2. The two predicted aromatic Gating residues, Y319 and F319, of the Gate-domains are boxed. Note the presence of H316 in isoform 1 and of L316 in isoform 2.

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