Figure 5

FGF2 treatment promotes a transient increase in association of Cx43 and phospho-PKCδ. Whole cell extracts were prepared from cells treated with vehicle or 5 ng/ml FGF2 for the indicated time. The extracts were immunoprecipitated (IP) with non-immune IgG, anti-Cx43, or anti-phospho-PKCδ (Thr505) antibodies, as indicated. A fraction of the input and the bead fractions from the immunoprecipitations were subjected to western blotting (WB) with anti-Cx43 antibodies. The co-precipitated Cx43 and phospho-PKCδ complex is most abundant at 10 minutes and returns to basal levels by 15 minutes. The graph reveals the average ratio of co-immunoprecipitated Cx43 relative to the Cx43 present in the input fraction for each time point from three separate experiments. Error bars indicate standard deviation. An asterisk denotes a p-value <0.05, relative to control. The ratio for the 0 min FGF2 treatment point has been arbitrarily set to a value of 1.