Figure 3
From: Interaction of connexin43 and protein kinase C-delta during FGF2 signaling
Co-detection of Cx43 and phospho-PKCδ at the plasma membrane. Serum starved MC3T3 cells were treated with FGF2 for 10 min prior to fixation and subsequent staining with (i) mouse anti-Cx43 (red), (ii) rabbit anti-phospho-PKCδ (Thr505) (green), (iii) DAPI (blue). A merged image is also shown (iv). Arrows indicate the same position on each panel and correspond to areas where both Cx43 and phospho-PKCδ appear to be closely co-detected in their respective panels. A representative field of view is shown. The white scale bar is ~10 μm.