Figure 2

FGF2 induces the accumulation of phospho-PKCδ in both the nuclei and plasma membrane. (A). Serum starved MC3T3 cells were subjected to immunofluorescent detection with anti-phospho-PKCδ (Thr505) antibodies during a time course of treatment with FGF2 (5 ng/ml). Representative images collected by epi-fluorescence microscopy are shown for each time point. Small arrows indicate the punctate staining at the cell periphery. The scale bar indicates ~10 μm. (B) Western blots (n = 3) probed with anti-PKCδ, anti-phospho-PKCδ (Thr505) and anti-Cx43 antibodies were performed on equal amounts of plasma membrane protein extracts from FGF2 (5 ng/ml) treated osteoblasts. Representative blots are shown.