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Table 2 Michaelis constants (Km) and maximal velocities (Vmax) of recombinant AzoB of P. kullae K24 expressed in E. coli.

From: Identification and molecular characterization of a novel flavin-free NADPH preferred azoreductase encoded by azoB in Pigmentiphaga kullae K24

Substrate

Km (μM)*

Vmax (U/mg protein)

NADPH

1.0

33

Orange I (NADPH)

3.0

36

NADH

170

17

Orange I (NADH)

8.6

22

  1. * Data were presented by the averages from triplicate with standard deviations of <10%;
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BMC Biochemistry

ISSN: 1471-2091

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